Abstract: Titanium-based high-entropy alloys with superior properties such as high specific strength and low density have received much attention from the military industry and scientific research community. However, how to find and obtain titanium-based high-entropy alloy materials with both strength and plasticity through improved processing, and how to establish a relationship between processing methods, material microstructure and mechanical properties still need in-depth research. In this study, a new high-entropy alloy Ti₄₅Zr₄₀Al₅Nb₅V₅ with a density of 5.6 g/cm³ was prepared by vacuum suspension melting, and the alloy was further strengthened and toughened by multi-stage rolling. It was found that multi-stage rolling induced the formation of B2 and HCP precipitated phases in Ti₄₅Zr₄₀Al₅Nb₅V₅ alloy. The combination of precipitated phases and fine grain strengthening synergistically increased the strength and plasticity of Ti₄₅Zr₄₀Al₅Nb₅V₅ material, and the tensile yield strength of the material was increased by 35.2% to 1,149 MPa, while the ductility of the material was increased by 34.0% to 10%.