Abstract: In view of the unavoidable phenomenon of fluid pulsation in the application of rotor pumps, a method for reducing fluid pulsation based on variable speed pumps is reported. In this study, the theoretical flow and speed simulation of the rotor pump were analyzed, and it was found that the pulsation of the rotor pump is related to the speed. To this end, a mathematical model of variable speed regulation was established according to the pulsation characteristics of the rotor pump, and the model was applied to the speed control of the rotor pump to reduce the fluid pulsation of the rotor pump. Firstly, the rotor pump with constant speed and variable speed regulation was simulated by Pumplinx software, and the evaluation of the simulation results was completed by using the fluid fluctuation evaluation index. Then, the experimental platform was built according to the actual conditions to conduct the experiments on the pump with constant speed and variable speed regulation. Finally, it shows that the method of reducing pulsation based on rotor pump speed regulation proposed in this study can effectively reduce the fluid pulsation of the rotor pump and improve transportation stability.