Abstract: The microstructures and tensile behavior,of the 3 D-printed,electron beam melted(EBM) Ti-6 Al-4 V alloy-plate(20 mm×10 mm×10 mm) with texture-gradient,were investigated with X-ray diffraction(XRD),scanning/transmission electron microscopy(SEM/TEM) and mechanical probes.Quite a few well-defined parallel “ribbons”,2.5 mm wide and formed with the lamellar layers,piled-up bottom up and comprising β-phased grains with different sizes and distributions,showed up,the boundary of which possibly originated from the thermal cycling of surface by e-beam.As the bottom-up height increased,the average size of β-phased grains rapidly-slowly increased,but the thickness of α-phased flakes rapidly decreased.As the height increased,the tensile strength increased from 842 to 895 MPa,accompanied by an increase of the elongation-at-break.A descending order of dislocation-defect density exists: the top》 the middle》 the bottom.Moreover,the anisotropic distributions of yield/tensile strengths and elongation-at-break were measured.